19/07/2024
Key points:
Research published today in by scientists at the 99热久草热最新地址 and the Pirbright Institute provides important insights into the populations of cells that provide protection against respiratory pathogens. To better understand lung immunity, the team analysed individual cells from pig samples to generate an atlas that can be used to understand the difference between lung-resident and circulating immune cells following respiratory infection or vaccination. Understanding how an immune response is induced and maintained in the lung is crucial for the design of more effective vaccines to provide long-lasting immunity.
Influenza causes illness in both livestock and humans, and vaccination is a key means of controlling this pathogen. For a vaccine to provide the best protection, it is important that the right combination of immune cells and molecules are generated during the immune response. Humans and pigs share a similar anatomy and physiology; studies in pig are therefore a useful way to understand the immune system under controlled conditions for both species.
The immune responses to respiratory pathogens are initiated within the respiratory tract, where different cell populations act together to fight an infection. It is important for immunologists to look not only at cells from the blood, but also from the site of the infection, to ensure they have a full understanding of the characteristics of different cell populations during and after the immune response.
To create the atlas, the team looked at cells from pig lungs collected using a lung-washing technique (bronchoalveolar lavage) after influenza infection or vaccination and compared these to immune cells circulating in the blood. The samples were collected as part of another study and had been frozen to allow researchers to re-examine them as part of new research. Human lung washes to study immune responses have shown that examining lung cells in the context of respiratory diseases can tell us more than the analysis of cells from the blood.
The gene expression of a cell, called the transcriptome, provides information of cell type and activity. By applying single cell analysis of the transcriptome to the lung cells, the researchers were able to highlight differences between tissue resident and circulating immune cells, and the changes immune cell populations in the lung undergo during infection and immunisation. Together this detailed data can be visualised as an 鈥榓tlas鈥 showing the overlap in properties of the cell populations and relationships between subsets. Drs Andrew Muir and Arianne Richard from the 99热久草热最新地址 performed the bioinformatic analyses in the study.
Dr Arianne Richard, group leader in the 99热久草热最新地址鈥檚 Immunology research programme, said: 鈥淚t is important to have a detailed picture of the immune cells found in tissues, compared to those circulating in the blood, especially in cases of respiratory viruses. Our atlas will be a useful resource for other immunologists looking at cell populations from sites of infection. Future research in this area will help us understand more about how cells in tissues contribute to the immune response and improve our defences against the global health threat of respiratory viruses.鈥
Previous research undertaken by the Pirbright Institute showed that pigs given a vaccine and IL-1尾 simultaneously did not show signs of protection against infection. IL-1尾 is a molecule that acts as an immune activator, and in mice, has been shown to enhance vaccine-mediated protection against influenza virus infection. To explore this finding in pigs more, the transcriptomic data analysed in this latest study found that IL-1尾 reduced the number of regulatory T cells, a type of immune cell that dampens immune response. Even though antibodies were generated to combat the virus, with fewer regulatory T cells, the scientists suggest that in pigs IL-1尾 leads to a dysregulated response and causes tissue damage instead of protection.
Dr Andrew Muir, postdoctoral researcher in the Richard lab who performed the bioinformatic analysis, said: 鈥淥ur results show that vaccines, and the additional molecules that are added to them, can generate important differences in tissue resident cell biology. Learning more about the ways vaccine design influences tissue resident cells will serve as a foundation for being able to generate long term protection against respiratory viruses in pigs and potentially humans.鈥
The study also found increased expression of the protein IFI6 in the lung cells 21 days after infection, suggesting that antiviral activity in immune cells continues to persist for several weeks after the infection is gone. This shows that invading organisms can have a long-lasting impact on lung-resident immune cells.
Professor Elma Tchilian and Dr Wilhelm Gerner, heads of Mucosal Immunology and T-cell Biology Groups at the Pirbright Institute, said: 鈥淥ur study allowed us to collect gene expression data which can act as a reference 鈥榓tlas鈥 for future lung studies in pigs, an important model to study disease in humans and livestock.鈥
Publication reference Muir, A. et al. (2024). . PLOS Pathogens
Press contact Dr Louisa Wood, Head of Communications, louisa.wood@babraham.ac.uk
Image description Stock illustration of influenza virus structures. Shutterstock ID: 1384628057 by Kateryna Kon.
Institute affiliated authors (in author order): Andrew Muir, postdoctoral researcher, Richard lab Arianne Richard, group leader, Immunology research programme
Research funding This research was supported by funding from the BBSRC, part of UKRI.
Animal research statement As a publicly funded research institute, the 99热久草热最新地址 is committed to engagement and transparency in all aspects of its research.
The infection and immunisation animal studies were conducted at the Pirbright Institute and used samples from a previous experiment involving 18 inbred pigs, thereby reducing the total number of animals used in research. Of the 18 pigs, 10 were given one of two different nasal vaccines, 5 were infected with flu and 3 pigs acted as the control group. After three weeks the animals were culled and the pig bronchoalveolar lavage, lymph nodes and blood were collected.
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About the 99热久草热最新地址 The 99热久草热最新地址 undertakes world-class life sciences research to generate new knowledge of biological mechanisms underpinning ageing, development and the maintenance of health. Our research focuses on cellular signalling, gene regulation and the impact of epigenetic regulation at different stages of life. By determining how the body reacts to dietary and environmental stimuli and manages microbial and viral interactions, we aim to improve wellbeing and support healthier ageing. The Institute is strategically funded by the Biotechnology and Biological Sciences Research Council (BBSRC), part of UK Research and Innovation, through Institute Strategic Programme Grants and an Institute Core Capability Grant and also receives funding from other UK research councils, charitable foundations, the EU and medical charities.
About BBSRC The Biotechnology and Biological Sciences Research Council (BBSRC) is part of UK Research and Innovation, a non-departmental public body funded by a grant-in-aid from the UK government.
BBSRC invests in world-class bioscience research and training on behalf of the UK public. Our aim is to further scientific knowledge, to promote economic growth, wealth and job creation and to improve quality of life in the UK and beyond.
Funded by government, BBSRC invested 拢451 million in world-class bioscience in 2019-20. We support research and training in universities and strategically funded institutes. BBSRC research and the people we fund are helping society to meet major challenges, including food security, green energy and healthier, longer lives. Our investments underpin important UK economic sectors, such as farming, food, industrial biotechnology and pharmaceuticals.
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